世界*實(shí)驗(yàn)材料供應(yīng)商 Viagen Biotech, Inc.正式授權(quán)上海起發(fā)為其中國代理， Viagen Biotech, Inc.在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 Viagen Biotech, Inc.就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Viagen Biotech Inc.是創(chuàng)新的DNA提取技術(shù)和生命科學(xué)研究產(chǎn)品的供應(yīng)商。ViagenDirectPCR®DNA提取系統(tǒng)是一種單管系統(tǒng)，用于從小鼠尾巴，耳朵片，卵黃囊和培養(yǎng)細(xì)胞中快速制備DNA。由Viagen Biotech Inc.的科學(xué)家開發(fā)的正在申請(qǐng)的組件允許得到的DNA提取物與用于基因分型的基因組PCR相容。生物樣品的粗提取物與許多分子生物學(xué)級(jí)反應(yīng)（如聚合酶鏈反應(yīng)（PCR））不相容，部分原因是粗提物中含有抑制劑。DirectPCR試劑不僅介導(dǎo)生物樣品的快速溶解，而且還含有有效抑制粗裂解物對(duì)PCR擴(kuò)增的抑制活性的抑制劑，同時(shí)zui大限度地保持了釋放的基因組DNA的完整性。該過程*消除了任何溶液轉(zhuǎn)移或開管步驟，節(jié)省了大量的時(shí)間和精力。DirectPCR試劑每尾只花費(fèi)26美分，可以節(jié)省任何使用量。

Viagenbiotech致力于簡便快速提取和制備核酸（DNA / RNA），以批量、快速進(jìn)行基因分析。公司擁有的技術(shù)不僅加快樣品的裂解，而且降低了粗提物中PCR抑制因素，因而使DNA提取與PCR基因分析兼容，樣品經(jīng)一步消化后即可進(jìn)行PCR擴(kuò)增，并且保證了基因組的完整性。

DirectPCR Lysis Reagent (Cell) 100ml

Availability: In stock

Quick Overview

Catalog No: 302-C

For use with cultured cells

Stable up to 12 months at 4℃ 

Ships at RT

Details

Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure compley eliminate any solution transfer or tube-opening steps, providing you with substantial extra time.

Brief procedure
1.  Lyse tails in DirectPCR® Lysis Reagent.
2.  Incubate for 45 min at 85°C.
3.  PCR genotyping with 1 μl lysates.

Detailed protocols:  Tail, Ear, Yolk Sac, and Cultured cells.

DirectPCR® system offers advantages over conventional protocols that include:
· Time saving: Less hands-on time. Crude tail lysates for PCR.
· Money saving: Cost-effective reactions.  
· Safe: No organic reagents. 
· Environmental: Less waste (organic reagents, tubes, tips, etc...) 
· Reliable and efficient: Virtually 100% success rate with high yields.

DirectPCR Lysis Reagent (cell)

301-C 50ml cultured cells

302-C 100ml cultured cells

部分產(chǎn)品報(bào)價(jià)如下：

我們公司zui大優(yōu)勢(shì)是強(qiáng)大的采購，

1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，

2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢(shì)，

3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免

4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢(shì)，當(dāng)然不能保證100%產(chǎn)品都是,因?yàn)橐馕吨鴽]有服務(wù).

5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大 交大 復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥 ，fisher等500多家公司

6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc.  Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等

7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。